Simple and Efficient Chromosome Doubling Method by Colchicine-treatment for Haploid Plantlets of Tobacco.
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چکیده
منابع مشابه
Simple and Efficient Method for the Preparation of 5-Bromoisatins
Isatin, N-methyl isatin and N-benzylisatin were smoothly monobrominated in the 5-position by treatment with pyridinium bromochromate (PBC) in acetic acid medium. In the similar conditions indigo underwent bromination and oxidation to give 5-bromoisatin in high yield.
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15 صفحه اولChromosome Doubling of Microspore-Derived Plants from Cabbage (Brassica oleracea var. capitata L.) and Broccoli (Brassica oleracea var. italica L.)
Chromosome doubling of microspore-derived plants is an important factor in the practical application of microspore culture technology because breeding programs require a large number of genetically stable, homozygous doubled haploid plants with a high level of fertility. In the present paper, 29 populations of microspore-derived plantlets from cabbage (Brassica oleracea var. capitata) and brocc...
متن کاملEfficient Doubled Haploid Production in Brassica napus via Microspore Colchicine Treatment in vitro and Ploidy Determination by Flow Cytometry
An efficient microspore culture protocol was used to rapidly produce homozygous doubled haploid (DH) progeny of crosses between low erucic canola and high erucic resynthesized rapeseed (Brassica napus L.). Microspores of Canadian cultivars ‘Excel’ and ‘Profit’ as well as three F1 hybrids with the resynthetic line RS239 were treated with colchicine immediately after isolation. Flow cytometry was...
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ژورنال
عنوان ژورنال: Japanese Journal of Breeding
سال: 1993
ISSN: 0536-3683,2185-291X
DOI: 10.1270/jsbbs1951.43.603